Lane 6 : HEK-293 (Human) Whole Cell Lysate ( ab52256) with Human alpha Tubulin peptide ( ab23537) at 1 µg/ml Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab27252) with Human alpha Tubulin peptide ( ab23537) at 1 µg/ml Lane 4 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate ( ab50957) Lane 3 : NIH/3T3 whole cell lysate ( ab7179) Lane 2 : HEK-293 (Human) Whole Cell Lysate ( ab52256) Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab27252) This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.Īll lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.Īcetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. ![]() Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. ![]() This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some glutamate residues at the C-terminus are polyglutamylated.
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